Cyclic ADP-ribose increases Ca2+ removal in smooth muscle

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Cyclic ADP-ribose increases Ca2+ removal in smooth muscle.

Ca2+ release via ryanodine receptors (RyRs) is vital in cell signalling and regulates diverse activities such as gene expression and excitation-contraction coupling. Cyclic ADP ribose (cADPR), a proposed modulator of RyR activity, releases Ca2+ from the intracellular store in sea urchin eggs but its mechanism of action in other cell types is controversial. In this study, caged cADPR was used to...

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Cyclic ADP - ribose increases Ca 2 + removal in smooth muscle Karen

Ca 2+ release from the sarcoplasmic reticulum (SR) via the ryanodine receptor (RyR) regulates both general cellular activities such as metabolism and more specific events such as smooth muscle contraction. RyR opening is itself promoted by Ca 2+ (Endo et al., 1970; Fabiato, 1983) and decreased by FK506 binding proteins (FKBPs) (Brillantes et al., 1994; Xin et al., 2002). Among other substances,...

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The purpose of the present study was to determine whether cyclic ADP-ribose (cADPR) acts as a second messenger for Ca2+ release through ryanodine receptor (RyR) channels in tracheal smooth muscle (TSM). Freshly dissociated porcine TSM cells were permeabilized with β-escin, and real-time confocal microscopy was used to examine changes in intracellular Ca2+ concentration ([Ca2+]i). cADPR (10 nM-1...

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Regulation of KCa-channel activity by cyclic ADP-ribose and ADP-ribose in coronary arterial smooth muscle.

The enzymatic pathway responsible for the production and metabolism of cyclic ADP-ribose (cADP-R) in small bovine coronary arteries was characterized, and the role of cADP-R and ADP-ribose (ADP-R) in the regulation of the activity of large-conductance Ca2+-activated K+(KCa) channels was determined in vascular smooth muscle cells (SMC) prepared from these vessels. We found that cADP-R and ADP-R ...

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Cyclic ADP-ribose contributes to contraction and Ca2+ release by M1 muscarinic receptor activation in coronary arterial smooth muscle.

The present study determined the role of cyclic ADP-ribose (cADPR) in mediating vasoconstriction and Ca(2+) release in response to the activation of muscarinic receptors. Endothelium-denuded small bovine coronary arteries were microperfused under transmural pressure of 60 mm Hg. Both acetylcholine (ACh; 1 nmol/L to 1 micromol/L) and oxotremorine (OXO; 2.5-80 micromol/L) produced a concentration...

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ژورنال

عنوان ژورنال: Journal of Cell Science

سال: 2003

ISSN: 1477-9137,0021-9533

DOI: 10.1242/jcs.00713